Study on factors affecting the remediation of DDT-contaminated soil using the Biosoil bio-preparation
34 viewsDOI:
https://doi.org/10.54939/1859-1043.j.mst.113.2026.120-126Keywords:
DDT-contaminated soil; Biosoil; Bio-preparation.Abstract
This study evaluates the remediation potential of the bioproduct Biosoil containing Bacillus subtilis for DDT-contaminated soil and identifies factors affecting treatment efficiency. Two field soil samples with initial DDT concentrations of approximately 80 mg/kg (MĐ-TXL-01) and 120 mg/kg (MĐ-TXL-02), exceeding the limits specified in QCVN 03:2023/BTNMT, were investigated. Experiments were conducted with straw supplementation to improve soil porosity, while the effects of temperature, product dosage, and treatment time were assessed. Isothermal conditions showed higher efficiency; however, variable-temperature conditions were selected for practical application. A 3% product dosage was optimal in terms of both technical performance and cost. DDT concentrations decreased rapidly within the first 5÷20 days, followed by a slower degradation rate. After 20 days of treatment with 3% Biosoil under variable-temperature conditions, MĐ-TXL-01 achieved concentrations below the permissible limit, while MĐ-TXL-02 remained above the threshold. The results demonstrate the potential of Bacillus subtilis-based Biosoil for DDT soil remediation; however, additional measures such as nutrient supplementation, soil turning, or supporting technologies are needed for highly contaminated soils.
References
[1]. R. Bidlan and H. K. Manonmani, “Aerobic degradation of dichlorodiphenyltrichloroethane (DDT) by Serratia marcescens DT-1P”, Process Biochemistry, Vol. 38, No. 1, pp. 49–56, (2002). DOI: https://doi.org/10.1016/S0032-9592(02)00066-3
[2]. J. A. Bumpus and S. D. Aust, “Biodegradation of environmental pollutants by the white rot fungus Phanerochaete chrysosporium”, Applied and Environmental Microbiology, Vol. 53, No. 9, pp. 2001–2008, (1987). DOI: https://doi.org/10.1128/aem.53.9.2001-2008.1987
[3]. N. V. Tai, C. T. Phong, N. K. Hung and P. N. Quan, “Treatment methods for Agent Orange/Dioxin in soil and sediment”, Journal of Military Science and Technology, Vol. 76, pp. 167–171, (2021).
[4]. Agency for Toxic Substances and Disease Registry, “Toxicological Profile for DDT, DDE, and DDD”, U.S. Department of Health and Human Services, Public Health Service, Atlanta, Georgia, (2002).
[5]. L. Juhasz and R. Naidu, “Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: A review of the microbial degradation of benzo1.[a]pyrene”, International Biodeterioration & Biodegradation, Vol. 45, No. 1–2, pp. 57–88, (2000). DOI: https://doi.org/10.1016/S0964-8305(00)00052-4
[6]. K. Singh, A. Walker, J. A. W. Morgan and D. J. Wright, “Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils”, Applied and Environmental Microbiology, Vol. 70, No. 8, pp. 4855–4863, (2004). DOI: https://doi.org/10.1128/AEM.70.8.4855-4863.2004
[7]. J. P. Verma, D. K. Jaiswal and R. Sagar, “Pesticide relevance and their microbial degradation: A-state-of-art”, Reviews in Environmental Science and Biotechnology, Vol. 13, pp. 429–466, (2014). DOI: https://doi.org/10.1007/s11157-014-9341-7
[8]. X. Li, J. He and S. Li, “Isolation of a novel DDT-degrading bacterium and its biodegradation pathway”, Environmental Science and Pollution Research, Vol. 26, pp. 20804–20814, (2019).
[9]. Y. Gao, X. Yang, H. Huang and Y. Zhu, “Microbial consortium for bioremediation of persistent organic pollutants: Current advances and future perspectives”, Journal of Environmental Management, Vol. 270, p. 110863, (2020).
[10]. Sharma, T. Satyanarayana and B. N. Johri, “Role of biosurfactants in bioremediation of persistent organic pollutants”, Critical Reviews in Biotechnology, Vol. 38, No. 2, pp. 259–278, (2018).
[11]. P. J. Strong and H. Claus, “Laccase: A review of its past and future in bioremediation”, Critical Reviews in Environmental Science and Technology, Vol. 41, No. 4, pp. 373–434, (2011). DOI: https://doi.org/10.1080/10643380902945706
[12]. X. Pan, D. Lin, Y. Zheng, Q. Zhang, Y. Yin, L. Cai, H. Fang and Y. Yu, “Biodegradation of DDT by Stenotrophomonas sp. DDT-1: Characterization and genome functional analysis”, Scientific Reports, Vol. 6, p. 21332, (2016). DOI: https://doi.org/10.1038/srep21332
[13]. T. P. Baczynski, D. Pleissner and T. Grotenhuis, “Anaerobic biodegradation of organochlorine pesticides in contaminated soil - Significance of temperature and availability”, Chemosphere, Vol. 78, No. 1, pp. 22–28, (2010). DOI: https://doi.org/10.1016/j.chemosphere.2009.09.058
[14]. D. T. Uyen, N. T. Huong, D. X. Hoai and N. H. Dung, “Research for selection of soil washing solvent and technology for remediation of heavy metal contaminated soil in chemical incident response”, Journal of Military Science and Technology, Vol. 77, pp. 67–72, (2022).
