Impact of ascorbic acid concentration on the survival rate of saccharomyces cerevisiae under gamma irradiation
DOI:
https://doi.org/10.54939/1859-1043.j.mst.110.2026.143-149Keywords:
Ascorbic acid; Saccharomyces cerevisiae; Gamma irradiation; Radiation protection.Abstract
Ionizing radiation induces severe oxidative stress, leading to cytotoxicity. This study investigates the radioprotective mechanism of Ascorbic Acid (AA) on Saccharomyces cerevisiae exposed to Cobalt-60 gamma irradiation. Yeast cells were treated with AA concentrations (300 µM) and subjected to radiation doses ranging from 25 to 100 Gy. Survival rates were quantified via colony formation assays and analyzed in comparison with previous studies on DNA single-strand breaks (SSB). While prior research demonstrated that AA consistently mitigates DNA damage, the current cellular results reveal a dose-dependent limitation. Specifically, 300 µM AA exhibited significant radioprotective efficacy at a low dose (25 Gy). However, at higher doses (75 - 100 Gy), AA paradoxically reduced survival rates compared to controls, diverging from its protective trend on DNA. The data may suggest a trend towards a functional transition of AA from an antioxidant to a pro-oxidant role, particularly when combined with high radiation doses. Consequently, the study implies that radioprotection observed at the molecular DNA level might not necessarily guarantee cellular survival under extreme oxidative conditions.
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