Determination of ofloxacin antibiotic residues in wastewater by high performance liquid chromatography with mass spectrometry (LC-MS)
Keywords:
Ofloxacin, LC-MS, Medical wastewater, Antibiotic residuesAbstract
A sensitive and selective liquid chromatographic method incorporating mass spectrometry has been developed for the determination of ofloxacin in medical wastewater over a concentration range of 0.1-80 µg/L. The study conducted to optimize the conditions for liquid-liquid extraction and solid phase extraction, followed by method validation parameters which were also determined through samples fortifying standards at important concentrations. The study used an Agilent HPLC system (Model 1200series) equipped with an Agilent 6130 Series Quadrupole LC/MS system with a Zorbax SB-C18 column (5 µm; 150 mm × 4.6 mm). The program is run with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid (50:50, v/v), at a flow rate of 0.5 mL/min, sample injection volume 10 µL. The results show that the method has accuracy from 70.05% 115.56%, detection limit of 0.2 ppb and limit of quantification from 0.6 ppb. Analysis of medical wastewater samples from the hospital after treatment showed that ofloxacin antibiotic residues were detected in concentrations ranging from 5.58 ± 0.31 ppb to 19.95 ± 1.22 ppb. The developed procedure is feasible for the determination of residues of ofloxacin in medical wastewater.
References
Alaton I.A., Dogruel S., Baykal E., Gerone G., Combined chemical and biological oxidation of penicillin formulation effluent, Journal of Environmental Management, 73(2) (2004), 155–163.
Guerra P., Kim M., Shah A., Alaee M., Smyth S.A., Occurrence and fate of antibiotic, analgesic/antiinflammatory, and antifungal compounds in five wastewater treatment processes, Science of the Total Environment, 473–474 (2014), 235–243.
Duong Hong Anh , Pham Ngoc Ha et al., Occurrence, fate and antibiotic resistance of fluoroquinolone antibacterials in hospital wastewaters in Hanoi, Vietnam, Chemosphere, 72(6) (2008), 968–973
Cañada-Cañada F., Espinosa-Mansilla A., Jiménez Girón A., Muñoz de la Peña A., Simultaneous Determination of the Residues of Fourteen Quinolones and Fluoroquinolones in Fish Samples using Liquid Chromatography with Photometric and Fluorescence Detection, Czech Journal of Food Sciences, 30(1) (2012), 74–82.
Ke He, Lee Blaney, Systematic optimization of an SPE with HPLC-FLD method for fluoroquinolone detection in wastewater, Journal of Hazardous Materials, 282 (2014), 96-105.
Fang P.F., Cai H.L., Li H.D. et al., Simultaneous determination of Isoniazid, Rifampicin, Levofloxacin in mouse tissues and plasma by high performance liquid chromatographytandem mass spaectrometry, Journal of Chromatography B, 878(24) (2010), 2286-2291.
Sun H., Wang H., Ge X., Simultaneous determination of the combined drugs of ceftriaxone sodium, metronidazole, and levofloxacin in human urine by high-performance liquid chromatography, International Journal of Science Innovations and Discoveries, 26(6) (2012), 486-492.
Detlef A. Bohm & Carolin S. Stachel & Petra Gowik, Validation of a multiresidue method for the determination of several antibiotic groups in honey by LCMS/MS, Anal Bioanal Chem, 403 (2012) , 2943–2953.
Official Journal of the European Communities. "Implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results". Commission Decision 2002/657/EC.
La Thi Quynh Lien, Nguyen Quynh Hoa, Nguyen Thi Kim Chuc et al., Antibiotics in Wastewater of a Rural and an Urban Hospital before and after Wastewater Treatment, and the Relationship with Antibiotic Use—A One Year Study from Vietnam, International Journal of Environmental Research and Public Health, 13(6) (2016), 558.
