OPTIMIZING THE EXTRACTION PROCESS OF ALKALOID FROM THE LEONURUS JAPONICAS HOUTT

405 views

Abstract

The purpose of this sudy was to investigate the one way solvent type, solvent concentration, extraction time and the ratio of material/solvent extraction, which affected the extraction process of alkaloid from Leonurus japonicas Houtt. resulting in C₂H₅OH; 80%; 24 hours; 6/1 (v/w). On the basis of the factors affecting the extraction conditions, we found that the solvent concentration, extraction time and solvent/material ratio strongly influence the extraction process. Application of Box-Behnken design found the optimal condition for the extraction process of alkaloid from Leonurus japonicas Houtt. With solvent concentration of 77.81%, extraction time of 24,24 hours, solvent/raw ratio whether it is 5.93/1 (v/w), which created the amount of alkaloid of 2.0996 g. Experimental results showed high degree of compatibility with the model.